The Board of Trustees may from time to time on its own motion or on petition amend, supplement or repeal the regulations, restrictions and boundaries herein established, but no such amendment, supplement or repeal shall become effective until after a public hearing in relation thereto shall have been held and all parties in interest and citizens shall have had an opportunity to be heard. Whenever the owners of 50% or more of the frontage in any proposed new district shall present to the Board of Trustees a petition duly signed and acknowledged requesting an amendment of this chapter, it shall be the duty of the Board of Trustees to hold a public hearing thereon and cause notice to be given as required.